Frequently Asked Question
Bacterial DNA Extraction Kit
Make sure that you use the recommended volume of bacterial culture for extraction. The culture should be fresh or properly stored one. Also ensure that the right amount of Lysis buffer and Proteinase K are added.
Lower culture density – make sure that the culture OD reaches 1.0 before processing the experiment.
- The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
- Improper storage and age of sample – Avoid using too old bacterial culture or culture stored at suboptimal conditions. Sheared DNA may be obtained from the old bacterial samples.
- Fresh samples are recommended for maximum genomic DNA yield
- Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted DNA
- Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.
- Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the DNA
- The eluted DNA smells ethanol.
- DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.
- DNA will not freeze well at -20℃
You can measure DNA by NanoDrop spectrophotometry or by fluorimeter using Qubit. In Qubit assay, the dye binds specifically to double stranded DNA and not to nucleotides like single-stranded DNA, or RNA.
Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to over estimation of DNA amounts due to the detection of contaminants in the sample.
The XpressDNA Bacteria kit works well with fresh and properly stored gram positive and gram-negative bacterial cultures.
Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.
No, you can directly go for downstream applications like PCR, restriction digestion, etc.,
Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.
The XpressDNA Bacteria kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.
The reagents present in this kit are guaranteed to be stable over a period of one year with the proper handling and storage condition.
Blood DNA Extraction Kit
Make sure that you use the recommended volume of properly stored blood for extraction. Also ensure that the right amount of Lysis buffer and Proteinase K are added.
- The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
- Improper storage of sample – Avoid using blood which is clotted or stored at suboptimal conditions.
- Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted DNA
- Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.
- Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the DNA
- The eluted DNA smells ethanol.
- DNA floats back from the well of agarose gels even if the DNA is loaded with loading dye.
- DNA will not freeze well in -20 ℃
You can measure DNA by NanoDrop spectrophotometry or by fluorimeter using Qubit. In Qubit assay, the dye binds specifically to double-stranded DNA and not to nucleotides, single-stranded DNA, or RNA.
Qubit assay specifically measures double-stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to overestimation of DNA amounts due to the detection of contaminants in the sample.
The XpressDNA Blood kit works well with fresh, old and /or haemolysed blood. The kit is ideal for isolating genomic DNA from blood samples stored in EDTA K2/K3, Heparin and Sodium fluoride storage vials.
No. This kit is not recommended for DNA extraction from blood clots.
Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in downstream process such as PCR, restriction digestion, etc.
The XpressDNA Blood kits are produced using a magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.
The reagents present in this kit are guaranteed to be stable over a period of one year.
Plasmid DNA Extraction Kit
Make sure that you take recommended volume of bacterial culture for extraction. The culture should be fresh. Also ensure that the exact amount of extraction buffer was added.
Make sure that the culture OD at 600nm has reached 1 – 1.5 before processing the experiment.
- The Plasmid DNA was handled improperly – Pipetting steps should be handled as gently as possible.
- Avoid too much of frothing while pipette mixing
- Do not vortex the assay tube at any stage of extraction
- Prolonged incubation at 80 ℃ while elution
- Improper storage and age of sample – Avoid using too old bacterial culture or culture stored at suboptimal conditions. Sheared DNA may be obtained from the old bacterial samples.
- Fresh samples are recommended for maximum plasmid DNA yield.
- Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted plasmid DNA
- Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.
- Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the plasmid DNA
- The eluted DNA smells ethanol.
- DNA floats back from the well of agarose gels even if the DNA is loaded with loading dye.
- DNA will not freeze well in -20 ℃
You can measure DNA by NanoDrop spectrophotometry
The XpressDNA Plasmid kit works well with fresh bacterial cultures containing clones.
Ensure that lysis incubation time is followed accurately, if prolonged incubation happened at 80°C during lysis time, will lead to genomic DNA contamination. Use fresh bacterial culture pellets for extraction.
Check whether the culture contains plasmid. Ensure that the cells are properly transformed.
Ensure that ethanol was added in the Wash buffer. Ensure the temperature of water bath is 80°C
Generally plasmid has three bands like supercoiled, linear and open circular forms. Ensure that the cells are properly transformed with single plasmid only. Avoid harsh pipette mixing while doing extraction, washing and elution. Avoid very old cultures
Yes, the Plasmid DNA can be used for sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.
No, you can directly go for downstream applications like PCR, restriction digestion, transformation etc.,
Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.
The XpressDNA Plasmid kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of plasmid DNA is higher because of the increased surface area of the nanoparticles.
The reagents present in this kit are guaranteed to be stable over a period of one year under the proper handling and storage condition.
Saliva DNA Extraction Kit
Make sure that you use the recommended volume of saliva for extraction. The saliva should be fresh or properly stored at -200˚C. Also ensure that the correct amount of Lysis buffer and Proteinase K are added.
- The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
- Make sure that saliva treated with stabilization buffer properly.
- Improper storage and age of sample – Avoid using too old saliva or stored at suboptimal conditions. Sheared DNA may be obtained from the old samples.
- Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted DNA.
- Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.
- Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the DNA
- The eluted DNA smells ethanol.
- DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.
- DNA will not freeze well at -20 ℃
You can measure DNA by NanoDrop spectrophotometry or by fluorimeter using Qubit. In Qubit assay, the dye binds specifically to double stranded DNA and not to nucleotides like single-stranded DNA, or RNA.
Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to over estimation of DNA amounts due to the detection of contaminants in the sample.
The XpressDNA Saliva kit works well with fresh and properly stored saliva at -200℃
Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.
No, you can directly go for downstream applications like PCR, restriction digestion, etc.,
Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.
The XpressDNA Saliva kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.
The reagents present in this kit are guaranteed to be stable over a period of one year with the proper handling and storage condition.
Soil DNA Extracton kit
- The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
- Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted DNA
- Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.
- Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the DNA
XpressDNA soil kit offers high lysis efficiency with a wide range of soil samples. The kit utilizes a combination of chemical and mechanical lysis methods to break open cell walls and membranes of different microbial species. In addition to bacterial samples, we have also tested fungi samples in sequencing platforms.
XpressDNA soil kit is validated in more than 70 different type of soil samples. High yields of pure microbial DNA can be extracted from all soil types, including compost, clay and top soil.
- The eluted DNA smells ethanol.
- DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.
- DNA will not freeze well at -20℃
The kit uses two step inhibitor removal for effective elimination of humic acid and other inhibitors from environmental samples. The extracted high quality inhibitor free genomic DNA is fully compatible with downstream PCR amplifications.
PCR, qPCR, and next-generation sequencing.
Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.
No, you can directly go for downstream applications like PCR, restriction digestion, etc.,
You can measure DNA by NanoDrop spectrophotometry or by fluorimeter using Qubit. In Qubit assay, the dye binds specifically to double stranded DNA and not to nucleotides like single-stranded DNA, or RNA.
Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to over estimation of DNA amounts due to the detection of contaminants in the sample.
The XpressDNA Soil kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.
The reagents present in this kit are guaranteed to be stable over a period of one year with the proper handling and storage condition.
Stool DNA EXtraction kit
- The genomic DNA was handled improperly – Pipetting steps should be handled as gently as possible.
- Frequent freeze thaw of DNA – avoid frequent freeze thaw of eluted DNA
- Non sterile consumables usage – ensure that the pipettes are cleaned, vials and tips autoclaved properly. Otherwise, the DNA will degrade due to nuclease activity.
- Improper Agarose gel and buffers – make sure that the gel and buffers are prepared properly. Do not use the old or used Agarose gels for checking the DNA
The kit utilizes a combination of chemical and mechanical lysis methods to break open cell walls and membranes of different microbial species. In addition to bacterial samples, we have also tested fungi samples in sequencing platforms.
Please follow the protocol for sample stored in storage/ stabilization buffer.
- The eluted DNA smells ethanol.
- DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.
- DNA will not freeze well at -20℃
You can measure DNA by NanoDrop spectrophotometry or by fluorimeter using Qubit. In Qubit assay, the dye binds specifically to double stranded DNA and not to nucleotides like single-stranded DNA, or RNA.
Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to over estimation of DNA amounts due to the detection of contaminants in the sample.
The kit uses inhibitor removal precipitation step for effective elimination of inhibitors from stool samples. The extracted high quality inhibitor free genomic DNA is fully compatible with downstream PCR amplifications
Yes, the DNA can be used for library preparation and sequencing. The protocol is optimized to extract DNA without contaminating proteins and PCR inhibitors.
No, you can directly go for downstream applications like PCR, restriction digestion, etc.,
PCR, qPCR, and next-generation sequencing.
The XpressDNA Stool kits are produced using magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.
The reagents present in this kit are guaranteed to be stable over a period of one year with the proper handling and storage condition.
Plant DNA Extraction Kit
Make sure that you use the recommended quantity of plant tissue extraction. It is always preferable to homogenize the tissue using liquid nitrogen and also ensure that the right amount of Lysis buffer was added.
If leaf samples are used, it is preferable to use tender leaf tissues.
- The eluted DNA smells ethanol.
- DNA floats back from the well of Agarose gels even if the DNA is loaded with loading dye.
- DNA will not freeze well at -20 ℃
- The genomic DNA was handled improperly – Pipetting steps should be performed as gently as possible.
- Dry seed samples tend to have a shearing in the genomic DNA, which is specific to the sample.
- Improper storage of sample – Repeated freezing and thawing of stored samples should be avoided as this may lead to shearing.
- The sample is old – Sheared DNA may be obtained from the old tissue or cell samples. Fresh samples are recommended for maximum genomic DNA yield.
- Different plant parts including leaf, dry seed, germinated seed, stem, flower, root and fruit tissues could be used for DNA extraction using XpressDNA Plant Kit.
- XpressDNA Plant kit was validated by extracting DNA from almost 70 different plant species.
- If the samples are rich in polysaccharides or polyphenols, it is recommended to perform a rebinding step to get rid of the excess contaminants. (Carefully follow the instructions in the Quick reference card)
No, you can directly go for downstream applications like PCR, restriction digestion, etc.
XpressDNA Plant kit works on the patented technology by making use of magnetic nanoparticles based patented technology. The major advantages include lesser dependence on time-consuming steps like centrifugation, use of spin columns and multiple tube changes. The recovery of genomic DNA is higher because of the increased surface area of the nanoparticles.
Yes. Avoid using Tris-EDTA (TE) buffer as EDTA will inhibit the activity of enzymes used in a downstream process such as PCR, restriction digestion, etc.
The reagents present in this kit are guaranteed to be stable over a period of one year.
DNA can be measured by Nanodrop spectrophotometry or by fluorimeter using Qubit. In Qubit assay, the dye binds specifically to double-stranded DNA and not to nucleotides, single-stranded DNA, or RNA.
Qubit assay specifically measures double stranded DNA and therefore provides a more accurate detection of DNA concentration and amount. NanoDrop spectrophotometry measures DNA concentration by determining the absorbance of light at 260 nm. Though double-stranded DNA has an absorbance at this wavelength, other molecules also absorb including proteins, RNA, single-stranded DNA, free nucleotides, phenol, and other contaminants. Therefore, NanoDrop measurements are prone to over estimation of DNA amounts due to the detection of contaminants in the sample.
